3,6-dihydroxyflavone decreases anti-oxidative enzyme activities in human leukemia HL-60 cells via MAPKs signaling pathway
- VernacularTitle:3,6-二羟黄酮降低白血病细胞HL-60抗氧化酶活性并影响MAPKs信号通路
- Author:
Hui LIN
;
Mantian MI
;
Hui CHANG
- Publication Type:Journal Article
- Keywords:
3,6-dihydroxyflavone;
HL-60 cells;
anti-oxidative enzyme;
MAPKs pathway
- From:Journal of Third Military Medical University
2003;0(10):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the intracellular mechanisms by which 3,6-dihydroxyflavone inducing apoptosis of HL-60 cells.Methods After the HL-60 cells were treated with 3,6-dihydroxyflavone at the concentration of 5.10,20 or 40 mol/L,the cells then were inoculated under the selected optimal concentration.The survival rate of HL-60 cells was analyzed using a haemocytometer with standard trypan blue dye exclusion and a CASY cell counter and analyzer.The contents of malondialdehyde(MDA)and the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GSH-Px)in the cells were measured by chemochromatometry.The expression of phosphorylation of ERK,JNK and p38MAPK were examined by Western blotting.Results The survival rate of HL-60 cells was significantly reduced in a dose-and time-dependent manner after 3,6-dihydroxyflavone treatment.When the dose was over 10 mol/L or the time was longer than 12 h under the concentration of 20 mol/L,significantly decreased survival rate was observed(P
