Expression of IL-8 induced by Pyocyanin in NCI-H292 cells by PKC-NF-?B signal pathway
- VernacularTitle:绿脓菌素通过PKC-NF-?B信号传导通路诱导人NCI-H292细胞表达IL-8
- Author:
Wenshu CHAI
;
Xiuli YAN
;
Fengqin WEI
;
Min WANG
- Publication Type:Journal Article
- Keywords:
pyocyanin;
NCI-H292 cell;
protein kinase C;
NF-?B;
IL-8
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study IL-8 expression of human airway epithelium-like NCI-H292 cells directly induced by Pyocyanin and its mechanism through protein kinase C(PKC) and nuclear factor-kappa B(NF-??).Methods ELISA methods were performed to test the expression of IL-8 in NCI-H292 cells infected by Pyocyanin.Western blot was employed to examine the expression of NF-?B protein.In addition,NCI-H292 cells were cultured together with PKC inhibitor,calphostin C or NF-?? inhibitor,pyrrolidine dithiocarbamate(PDTC),respectively before Pyocyanin infection,then the expression of IL-8 and NF-?? was assayed using the above methods.Results Pyocyanin was able to induce IL-8 protein secretion in NCI-H292 cells and had marked concentration-dependent relations.Pyocyanin could also significantly promote the activation of NF-?B,which peaked at 60~90 min.PDTC and calphostin C could significantly decrease the activation of NF-?? and the expression of IL-8.Conclutions Pyocyanin can induce IL-8 production.The direct induction of Pyocyanin can promote the activation of NF-?B by PKC signal pathway,then cause the expression and secretion of IL-8.
