Protective effect of SMT on acute cerebral infarction
- VernacularTitle:S-甲基异硫脲对急性缺血性脑卒中的保护作用
- Author:
Jian ZHANG
;
Min ZHANG
- Publication Type:Journal Article
- Keywords:
SMT;
cerebral infarction;
ESR;
standard addition method;
NO;
microglia
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To explore the protective effect of SMT on acute cerebral infarction.Methods Focal cerebral infarction was induced by photochemistry.The expression of iNOS was detected in microglia by double fluorescent immunohistochemistry and laser scanning confocal microscopy. NO content at post-ischemic time points was determined by electron spin resonance(ESR) technique and standard addition method.TTC stain was used to determine the infarct volume.Results At ischemic early period,compared with control group,NO content was not remarkably different after 2 h,6 h of ischemia in the treatment groups,infarct volumes also were not obviously changed.At ischemia mid and late period 12 h,24 h,48 h,the expression of iNOS and NO content was suppressed by SMT treatment in microglia.In TTC stain results,infarct volumes were increased obviously after 12 h,and achieved the peak after 24 h,48 h in control groups,infarct volumes in treatment groups were significantly decreased after 12 h,24 h,48 h.Conclusion SMT may play a protective role in acute cerebral infarction by reducing the expression of iNOS and NO content in microglia.
