Influences of shRNA on survivin gene expression and sensitivity of A549 cells to paclitaxel
- VernacularTitle:shRNA对A549细胞survivin基因表达及紫杉醇敏感性的影响
- Author:
Junhong DONG
;
Zhenming WANG
;
Xinhua FU
;
Shouxun WANG
;
Huansheng HUANG
- Publication Type:Journal Article
- Keywords:
shRNA;
survivin;
paclitaxel;
lung adenocarcinoma cells
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To construct the eukaryotic expression vectors of short hairpin RNA targeting survivin and observe its effect on biologic behavior of A549 cells and sensitivity of A549 cells to paclitaxel.Methods The DNA fragment targeting human survivin was inserted into the plasmid,and the recombinant plasmid was constructed.The recombinant plasmids cells were transfected into A549 cells by FuGENE transfection reagent.The expression levels of survivin gene were detected with RT-PCR and Western blot before and after transfection,respectively.Cell apoptosis was detected by TUNEL method.The sensitivity of A549 cells to paclitaxel was detected by MTT after transfection.Results The recombinant plasmid was successfully constructed.RNAi group cells showed lower expression of survivin than control group.The apoptosis rate of A549 cells increased after transfection.The IC50 of paclitaxel inhibiting A549 cells was 11.9 fold before transfection compared with those after transfection.There was significant difference between the two groups(P
