Effects of recombinant human endostatin on cell cycle and PCNA expression in fibroblast-like synoviocytes in rats with adjuvant arthritis
- VernacularTitle:重组人内抑素对佐剂性关节炎大鼠成纤维样滑膜细胞周期和PCNA表达的影响
- Author:
Xueying HUANG
;
Feihu CHEN
;
Xiaoming ZHANG
;
Yongjing LIU
;
Fenglai YUAN
- Publication Type:Journal Article
- Keywords:
endostatin;
adjuvant arthritis;
cell cycle;
proliferating cell nuclear antigen;
fibroblast-like synoviocytes
- From:
Chinese Pharmacological Bulletin
1986;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the effects of recombinant human endostatin(rhEndostatin) on cell cycle and the expression of proliferating cell nuclear antigen(PCNA) in fibroblast-like synoviocytes in rats with adjuvant arthritis(AA),and to explore the molecular mechanisms of the inhibitory effect of rhEndostatin on proliferation of fibroblast-like synoviocytes(FLS) in AA rats.Methods Adjuvant arthritis was induced by Freund′s complete adjuvant in rats.Flow cytometry was applied to measure the effects of rhEndostatin on the cell cycle in AA FLS.The effect of rhEndostatin on the expression of PCNA mRNA and protein in synovial tissue in AA rats was examined quantitatively by real-time fluorescent quantitative PCR and Western blot assays,respectively.Results The percentage of cells in G1 phase in AA FLS decreased significantly(P
