Effects of ?-lipoic acid on cerebral IRE1 expression induced by sleep deprivation in rats
- VernacularTitle:?-硫辛酸对睡眠剥夺所致的大鼠脑中IRE1表达的影响
- Author:
Jing FENG
;
Zhongxin ZHAO
- Publication Type:Journal Article
- Keywords:
sleep deprivation;
IRE1;
endoplasmic reticulum;
hippocampus;
?-lipoic acid;
oxidative stress
- From:
Academic Journal of Second Military Medical University
1985;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the influences of sleep deprivation and antioxidant agent ?-lipoic acid on the expression of IRE1 in the cortex and hippocampus in rats.Methods:Rats were randomly divided into control groups and sleep deprivation group.The control groups included blank control group(n=10) and environmental control group(n=10).Sleep deprivation group included 120 rats,and rats were further divided into ?-lipoic acid group(25 mg?kg-1?d-1) and sleep deprivation model group.The two were further divided into 6 groups according to the periods of sleep deprivation and sleep restoration(SD 1 d,SD 3 d,SD 5 d,SD 7 d,SD 7 d/RS 6 h,SD 7 d/RS 12 h[n=10]).The expression of IRE1 mRNA and protein was examined by RT-PCR and immunohistochemistry,respectively.Results:RT-PCR results showed that the expression of IRE1 mRNA in the cortex and hippocampus were significantly higher in sleep deprivation group than in the blank and environmental control groups(P
