Expression of peroxisome proliferator-activated receptor (PPAR)-alpha and PPAR-gamma in the lung tissue of obese mice and the effect of rosiglitazone on proinflammatory cytokine expressions in the lung tissue.
10.3345/kjp.2013.56.4.151
- Author:
Seung Lok RYU
1
;
Jae Won SHIM
;
Duk Soo KIM
;
Hye Lim JUNG
;
Moon Soo PARK
;
Soo Hee PARK
;
Jinmi LEE
;
Won Young LEE
;
Jung Yeon SHIM
Author Information
1. Department of Pediatrics, Kangbuk Samsung Hospital, Sungkyunkwan University School of Medicine, Seoul, Korea. jy7.shim@samsung.com
- Publication Type:Original Article
- Keywords:
Peroxisome proliferator-activated receptors;
Obesity;
Transforming growth factor-beta;
Vascularendothelial growth factor;
Tumor necrosis factor-alpha
- MeSH:
Adipokines;
Adiponectin;
Animals;
Bronchoalveolar Lavage;
Cell Count;
Cytokines;
Inflammation;
Leptin;
Lung;
Macrophages;
Mice;
Mice, Obese;
Neutrophils;
Obesity;
Ovalbumin;
Ovum;
Peroxisome Proliferator-Activated Receptors;
Peroxisomes;
PPAR alpha;
Real-Time Polymerase Chain Reaction;
Receptors, Leptin;
RNA, Messenger;
Thiazolidinediones;
Transforming Growth Factor beta;
Transforming Growth Factors;
Tumor Necrosis Factor-alpha;
Vascular Endothelial Growth Factor A
- From:Korean Journal of Pediatrics
2013;56(4):151-158
- CountryRepublic of Korea
- Language:English
-
Abstract:
PURPOSE: We investigated the mRNA levels of peroxisome proliferator-activated receptor (PPAR)-alpha, PPAR-gamma, adipokines, and cytokines in the lung tissue of lean and obese mice with and without ovalbumin (OVA) challenge, and the effect of rosiglitazone, a PPAR-gamma agonist. METHODS: We developed 6 mice models: OVA-challenged lean mice with and without rosiglitazone; obese mice with and without rosiglitazone; and OVA-challenged obese mice with and without rosiglitazone. We performed real-time polymerase chain reaction for leptin, leptin receptor, adiponectin, vascular endothelial growth factor (VEGF), tumor necrosis factor (TNF)-alpha, transforming growth factor (TGF)-beta, PPAR-alpha and PPAR-gamma from the lung tissue and determined the cell counts and cytokine levels in the bronchoalveolar lavage fluid. RESULTS: Mice with OVA challenge showed airway hyperresponsiveness. The lung mRNA levels of PPARalpha and PPAR-gamma increased significantly in obese mice with OVA challenge compared to that in other types of mice and decreased after rosiglitazone administeration. Leptin and leptin receptor expression increased in obese mice with and without OVA challenge and decreased following rosiglitazone treatment. Adiponectin mRNA level increased in lean mice with OVA challenge. Lung VEGF, TNF-alpha, and TGF-beta mRNA levels increased in obese mice with and without OVA challenge compared to that in the control mice. However, rosiglitazone reduced only TGF-beta expression in obese mice, and even augmented VEGF expression in all types of mice. Rosiglitazone treatment did not reduce airway responsiveness, but increased neutrophils and macrophages in the bronchoalveolar lavage fluid. CONCLUSION: PPAR-alpha and PPAR-gamma expressions were upregulated in the lung tissue of OVA-challenged obese mice however, rosiglitazone treatment did not downregulate airway inflammation in these mice.
