Upregulation of chemokine transcriptive levels induced by avian H5N1 and human H1N1 influenza viruses in mouse microglia and astrocytes
- VernacularTitle:流感病毒H1N1和H5N1感染小鼠小胶质细胞与星形胶质细胞诱导趋化因子转录水平上调
- Author:
Gefei WANG
;
Weizhong LI
;
Heng ZHANG
;
Jun ZENG
;
Dangui ZHANG
;
Youying CHEN
;
Xiaoxuan CHEN
;
Kangsheng LI
- Publication Type:Journal Article
- Keywords:
Microglia;
Astrocyte;
Influenza virus;
Chemokine
- From:
Chinese Journal of Immunology
2010;26(1):29-33,36
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the innate immune response of influenza virus-infected glial cells,the transcription levels in chemokines in mouse microglia and astrocytes were detected which pre-infected by human H1N1 or avian H5N1 influenza viruses.Methods: The glial cells isolated from neonatal mice cerebral cortex were cultured and further microglia and astrocytes were purified.The primary mouse microglia and astrocytes were infected in vitro by H1N1 or H5N1 influenza viruses in a multiplicity of infection (MOI) 2.Eight hours post infection,the influenza virus nucleoprotein (NP) was detected by immunofluorescence to identify the proportion of infected cells.The cellular RNA were extracted at 6 h and 24 h to detect the transcriptional level of chemokines by semi-quantitative RT-PCR.Results: More than 95% of the microgha and astrocytes which isolated from mice were infected.The transcription levels of CCL-3,CCL-5,CXCL-2,CXCL-9 and CXCL-10 from infected microglia and astrocytes were upregulated.Futhermore,the mRNA level of CXCL-10 increased much more.In addition,avian H5N1 influenza virus could induce more stronger upregulation of those chemokines than human H1N1 did.Conclusion: The mouse microglia and astro cytes which are infected by H1N1 influenza virus or H5N1 influenza virus could induce upregulation of transcription level of chemokines.
