HPV16E6 transfection improves growth and cell division of cervical carcinoma cell line C33A
- VernacularTitle:pcDNA3转染HPV16E6基因对人宫颈癌细胞C33A细胞生长及周期的影响
- Author:
Xiukun YANG
;
Xuannan CHEN
;
Zheng JIANG
- Publication Type:Journal Article
- Keywords:
human papilloma virus;
cervical carcinoma cells;
proliferation;
cell cycles
- From:Journal of Third Military Medical University
2003;0(09):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the transfection of human papilloma virus types 16E6 gene on the proliferation and apoptosis of cervical carcinoma cell line C33A. MethodsPlasmid pcDNA3-HPV16E6 was stably transfected into C33A cells by liposome, and the expression of HPV16E6 mRNA and protein in the transfected cells, the cells transfected with blank vector and the cells without transfection were identified by RT-PCR and Western blot analysis. The growth, proliferation and cell cycles of the 3 kinds of C33A cells were by cell growth curve plotting and flow cytometry analysis. ResultsC33A cells were transfected stably by liposome with plasmid pcDNA3-HPV16E6 (C33A-E6 cells)and plasmid pcDNA3 (C33A-P cells). The growth of C33A-E6 cells was more rapid than those of C33A-P and C33A cells (P0.05). ConclusionHPV16E6 is involved in the cell cycle probably through some signal pathways, and promotes cell division and proliferation of C33A cells.
