Mechanism of migration of vascular adventitial fibroblasts enhanced by advanced glycation end-products and the inhibition effect of candesartan
- VernacularTitle:晚期糖基化终产物促进血管外膜成纤维细胞迁移机制及坎地沙坦的干预作用研究
- Author:
Yayang LIU
;
Chun LIANG
;
Zonggui WU
- Publication Type:Journal Article
- Keywords:
glycosylation end products,advanced;
fibroblasts;
cell movement;
mitogen-activated protein kinases;
candesartan
- From:
Medical Journal of Chinese People's Liberation Army
1983;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the mechanism of migration of vascular adventitial fibroblasts(AFs) enhanced by advanced glycation end-products(AGEs) and the inhibition effect of candesartan.Methods Isolated vascular adventitial fibroblast of Sprague-Dawley(SD) rats were cultured.Migratory potential was estimated with Transwell chamber in vitro.Expression of receptor for AGEs(RAGE) and phosphorylated mitogen-activated protein kinase(MAPK) of AFs were determined by RT-PCR and Western blotting.Results AGEs modified human serum albumin(AGE-HAS,concentration ranged from 0 to 300mg/L) up-regulated the expression of RAGE at mRNA and protein levels,peaked at a concentration of 200mg/L(P
