Effect of the serum contained Radix Astragali,Hirudo,Hirudin and the compounding on the growth cycle and apoptosis of rats' glomerular mesangial cells
- VernacularTitle:黄芪、水蛭、水蛭素及水蛭黄芪配方含药血清对大鼠肾小球系膜细胞生长周期及凋亡的影响
- Author:
Xianzhi REN
;
Shumin JIANG
;
Wensheng ZHAI
- Publication Type:Journal Article
- Keywords:
Rat;
Glomerulum mesangial cells;
Growth cycle;
Apoptosis;
Morphology;
Radix Astragali;
Hirudo;
Hirudin
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate effect of the serum contained Radix Astragali, Hirudo, Hirudin and the compounding of these two herbs respectively on the growth cycle and apoptosis of rats' glomerular mesangial cells(GMCs)and observe the morphology of apoptosis. Methods: The growth cycle and apoptosis of GMCs was measured by flow cytometer. Meanwhile morphous of apoptosis was visualized by Wright's staining and observed by microscope. Results: The serum contained Radix Astragali, Hirudo, Hirudin and the compounding respectively can keep most GMCs in the stage of G0/G1,and it had a significant deviation compared with the control(P
