EFFECTS OF FOLIC ACID ON APOPTOSIS OF NEURAL CELLS IN RATS WITH FOCAL CEREBRAL ISCHEMIA INJURY
- VernacularTitle:叶酸对局灶性脑缺血大鼠神经细胞凋亡的影响
- Author:
Wenping DU
;
Guowei HUANG
;
Xumei ZHANG
;
Huan LIU
;
Guanglei WANG
- Publication Type:Journal Article
- Keywords:
folic acid;
focal cerebral ischemia;
apoptosis;
extracellular signal-regulated kinase(ERK)
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of folic acid on apoptosis of neural cells after focal cerebral ischemia injury in rats and the mechanisms.Method Thirty two adult male Sprague-Dawley(SD) rats were randomly divided into sham operation group(SO),middle cerebral artery occlusion group(MCAO),MCAO+ low dose folic acid group(MCAO+FA-L) and MCAO+ high dose folic acid group(MCAO+FA-H).The model of middle cerebral artery occlusion(MCAO) was set up by using intraluminal filament method.The rats were sacrificed at D7 day after cerebral ischemia.The apoptotic rate of neural cells was examined by TUNEL test,and the expression of pERK1/2 protein was detected by Western blotting method,The MDA content and serum SOD and GSH-Px activities in rats were measured before and 28d after folic acid treatment and 7th day after ischemia.Results Compared with ischemia group,the apoptotic rate of neural cells and MDA content in both folic acid supplemented groups were decreased significantly(P
