EFFECT OF HOMOCYSTEINE ON POTENTIATING ?ETA-AMYLOID NEUROTOXICITY
- VernacularTitle:同型半胱氨酸对A?神经毒性促进作用
- Author:
Yue HOU
;
Yan HONG
;
Weiqiang CHEN
;
Donglan WANG
;
Xizheng ZHANG
;
Yiyong CHENG
- Publication Type:Journal Article
- Keywords:
homocysteine;
?-amyloid;
hippocampal neurons;
apoptosis;
calcium influx;
oxidative stress
- From:
Acta Nutrimenta Sinica
1956;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the possibility that homocysteine(Hcy) may augment ?-amyloid(A?) neurotoxicity.Method Cultured hippocampal neurons were treatd with Hcy and/or A?42 and it's apoptosis,calcium influx,DNA damage and oxidative injury were examined.Results Combined treatment with 250?mol/L Hcy and 10?mol/L A?42 increased the apoptosis rate of hippocampal neurons significantly more than either agent alone,and even the sum of each agent treated alone.The combined treatment significantly exceeded the cytosolic calcium accumulation and MDA obtained with either alone or the sum of each agent.A significant increase in DNA damage also occurred in neurons exposed to A?42 in the presence of Hcy,but the magnitude of the increase was not greater than that seen with either treatment alone.Conclusion Hcy could potentiate A? neurotoxicity by neuron exitotoxicity,oxidative stress and inducing apoptosis.
