Effects of quercetin and psoralen on expression of ER?,ER? protein levels in T47D cells
- VernacularTitle:槲皮素、补骨脂素对人乳腺癌T47D细胞两种受体亚型表达的影响
- Author:
Lixia SHEN
;
Jianzhao NIU
;
Jifeng WANG
- Publication Type:Journal Article
- Keywords:
phytoestrogen;
genistein;
quercetin;
psoralen;
estrogen receptor
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the phytoestrogenic effects and mechanism on ER?,ER? expression levels at the protein of quercetin and psoralen in T47D cells.Methods T47D cells were treated with 10 ?mol?L-1 quercetin and 10 ?mol?L-1 psoralen for 48h respectively,total cell extracts examined for the levels of ER? and ER? protein by RT-PCR and Western blot analysis.The estrogen-like effect of quercetin and psoralen and their relations with the estrogen-receptor were evaluated by pure estrogen receptor antagonist ICI182,780 as a tool.Results Psoralen(10 ?mol?L-1) and quercetin(10 ?mol?L-1) up-regulated the expression of ER? mRNA and protein,without any effect on ER? mRNA and protein levels.The above up-regulation on ER? protein could be inhibited by adding estrogen receptor antagonism ICI182,780.Conclusion Psoralen and quercetin exerts estrogenic activity to ER-positive cells proliferation through interaction with ER? expression.
