Effect of darbufelone on the proliferation and apoptosis of SGC-7901 cell line
- VernacularTitle:darbufelone对胃癌SGC-7901细胞增殖、凋亡的影响
- Author:
Min PAN
;
Chunlun LIU
;
Xiaojie FANG
;
Jianhua SHI
- Publication Type:Journal Article
- Keywords:
SGC-7901 cells;
COX-2;
5-LOX;
proliferation;
apoptosis
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of dual COX-2/5-LOX inhibitor darbufelone on the inhibition of gastric adenocarcinoma in vitro.Methods MTT assay was used to detect the inhibition of gastric adenocarcinoma cell line SGC-7901 by darbufelone in different concentrations and at different times.TUNEL staining was used to evaluate the apoptosis of SGC-7901 cells.The changes of 5-LOX and COX-2 mRNA expression were studied by reverse transcription polymerase chain reaction.5-LOX and COX-2 protein expressions were analyzed by immunocytochemistry in SGC-7901 cells.Results darbufelone could decrease the proliferation significantly in a dose-dependent and time-dependent manner in SGC-7901 cells.After treating SGC-7901 with darbufelone at concentration of 1.5?10-5,1.0?10-5,5?10-6 mol?L-1 for 72 hs,apoptosis index(%) of SGC-7901 was(30.3?2.1)%,(23.0?2.0)%,(15.0?1.5)%,respectively,which was significantly higher than(0.6?0.1)% that in control group(P
