Effect of iptakalim on prevention isoprenaline induced cardiomyocytes hypertrophy
- VernacularTitle:埃他卡林对异丙肾上腺素诱导心肌细胞肥大的影响
- Author:
Shan GAO
;
Chaoliang LONG
;
Ruhuan WANG
;
Hai WANG
- Publication Type:Journal Article
- Keywords:
cardiomyocytes hypertrophy;
iptakalim;
isoprenaline;
laser confocal microscopy
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effect of iptakalim on neonatal cardiomyocytes hypertrophy induced by isoprenaline(ISO).Methods Hypertrophy in neonatal rat cardiac myocytes was established via culture with ISO.Total protein content was measured by Lowrys method.The fluorescence intensity of intracellular Ca2+ was detected respectively with Fluo-3/AM loading by the laser confocal microscopy.Results ① 1?10-5mol?L-1 ISO can activated ?-AR completely;② the total protein of cardiomyocytes and intracellular i was markedly decreased by treatment with IPT and represented a dose-dependent manner.Conclusion IPT could inhibit the increase of protein content of cardiomyocytes induced by ISO,which may be contributed to the decease of intracellular Ca2+ concentration.
