Identification of proteins associated with proliferation inhibition effects by DADS in SW480 cell line
- VernacularTitle:二烯丙基二硫抑制SW480细胞系生长增殖的相关蛋白质分子鉴定
- Author:
Qihui ZHAO
;
Qingchao QIU
;
Xiusheng HE
;
Bo HU
;
Qiao LUO
;
Changying LI
;
Yangchun LIU
- Publication Type:Journal Article
- Keywords:
diallyl disulfide;
colon carcinoma;
SW480 cell line;
Ubiquitin;
FKBP
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the arrest effect of diallyl disulfide(DADS) in the cell cycle of human nasopharyngeal carcinoma SW480 cell line and its molecular mechanism.Mothdes The growth inhibition effect of DADS of different concentrations on SW480 cell line was measured by MTT assay and cell counting.Phase distribution of cell cycle was analyzed by flow cytometry.Expression of Ubiquitin and FKBP was determined by Western blot.Results The MTT assay showed that DADS inhibited growth of SW480 cells significantly in a dose-dependent manner.Adding 25,35,50 and 70 mg?L-1 DADS for 48 hours,SW480 cell growth was suppressed by 18.67%,33.02%,49.12% and 66.86%,respectively.There were significant differences between the treated and controlled cases(P
