Effects of irbesartan on the expression of CTGF and MT1-MMP in high glucose-induced rat glomerular mesangial cells
- VernacularTitle:厄贝沙坦对高糖刺激的大鼠肾小球系膜细胞结缔组织生长因子及膜型基质金属蛋白酶-1表达的影响
- Author:
Fang YAO
;
Zhe YAN
;
Yonghong SHI
;
Jun HAO
;
Huijun DUAN
- Publication Type:Journal Article
- Keywords:
irbesartan;
glomerular mesangial cells;
connective tissue growth factor;
membrane-type 1 matrix metalloproteinase
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Ang Ⅱ receptor antagonist irbesartan on the expressions of connective tissue growth factor(CTGF) and membrane-type 1 matrix metalloproteinase(MT1-MMP) in high glucose-cultured rat glomerular mesangial cells(GMCs).Methods High concentration glucose and irbesartan were used to stimulate the cultured rat GMCs in vitro.The mRNA and protein expressions of CTGF and MT1-MMP were detected with semi-quantitative RT-PCR and Western blot.The secreted collagen Ⅳ in the supernatants of the GMCs was detected by enzyme-linked immunoadsorbent assay(ELISA).Results Compared with control group,the expressions of CTGF were continuously increased in GMCs under high concentration glucose medium;otherwise the mRNA and protein levels of MT1-MMP in GMCs were decreased in a time-dependent manner at the same time.These changes were accompanied by increased secretion of collagen Ⅳ.Irbesartan could inhibit those changes induced by high glucose.Conclusions High glucosecould induce the expression of CTGF and inhibit the expression of MT1-MMP in GMCs.Irbesartan could inhibit the secretion of ECM in GMCs under high concentration glucose medium,partly by regulating the expressions of CTGF and MT1-MMP.
