Transport and uptake of ecdysterone in Caco-2 cell monolayers
- VernacularTitle:蜕皮甾酮在Caco-2细胞模型中的摄取和跨膜转运研究
- Author:
Jiaoni ZHENG
;
Xiaohong LONG
;
Feng QIU
;
Zongyin QIU
- Publication Type:Journal Article
- Keywords:
ecdysterone;
Caco-2 cell monolayer;
1?,25-dihydroxyvitamin D3;
in vitro absorption
- From:
Chinese Pharmacological Bulletin
1986;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim This study examined the uptake and transport of ecdysterone(EDS) using Caco-2 cell monolayers as a model of human intestinal mucosa.Methods Two kinds of Caco-2 cell monolayer model(Caco-2 cell monolayers;CYP3A4 expressing Caco-2 cell monolayers) were set up to study the uptake and transport of EDS.Results Compared bi-directional charaterizaton of Caco-2 cell,the apparent permeability coefficients(Papp) values of EDS were between 0.1?10-6 cm?s-1 and 1?10-6 cm?s-1.EDS absorption was 1%~10% for two kinds of Caco-2 models.The RPapp values were all less than 1.5 for 4 h.Conclusions The uptake and transport of EDS was a passive transcellular diffusion mechanism.Ecdysterone was not influenced by CYP3A4 mediate mechanism.