A mouse model of acute lung inflammation induced by lipopolysaccharide inhalation
- VernacularTitle:吸入脂多糖诱导小鼠急性肺炎模型的建立
- Author:
Ling GUO
;
Wenjing LI
;
Mingjiang XU
;
Xian WANG
- Publication Type:Journal Article
- Keywords:
Lipopolysaccharides;
Pneumonia;
Models,animal;
Mice
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To develop a convenient,economical and stable model of acute lung inflammation in mice.Methods:BALB/c mice were inhaled intranasally with 50 ?L of LPS(1 g/L) or sterile PBS,and sacrificed at different time points after being anaesthetized.The bronchoalveolar lavage fluid(BALF) was collected,and the lungs were separated and homogenated or embedded and sliced to 5 ?m sections,which were then stained by HE to determine the severity of inflammation.The inflammatory cell infiltration in bronchoalveolar lavage was counted and IL-1?,the pro-inflammatory cytokine,measured by ELISA in lung homogenate and BALF.Results:The data showed that administration with 50 ?g of LPS(1 g/L) for 2 h resulted in significant inflammation in the lung.LPS mainly stimulated the recruitment of neutrophils within 24 h.And LPS was a quick revulsant of IL-1? production in BALF and in lung tissue between 4 and 24 h.Macrophages and lymphocytes recruited after 1 day,and sustained for at least 3 days.Conclusion:The results indicate that intranasal administration of LPS can induce a rapid and stable acute inflammatory model in mice.
