Interleukin 2 promotes the expression of immunoglobulin G in a cervical cancer cell line HeLaS3
- VernacularTitle:白细胞介素2促进宫颈癌细胞系HeLaS3免疫球蛋白G的表达
- Author:
Tian LU
;
Xiaohui ZHU
;
Shiqing LIU
;
Jie ZHENG
;
Xiaoyan QIU
- Publication Type:Journal Article
- Keywords:
Interleukin 2;
Uterine cervical neoplasms;
Immunoglobulin G;
Cell line,tumor
- From:
Journal of Peking University(Health Sciences)
2004;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To analyze the effect of interleukin 2(IL-2) on immunoglobulin G(IgG) expression in cervical cancer cell line HeLaS3.Methods:By immunofluorescence-flow cytometry(FCM),we detected membrane and cytoplasmic IgG expression variation of HeLaS3 after IL-2 stimulation.By western blot,we detected IgG expression variation of HeLaS3 after IL-2 stimulation.Meanwhile,by RT-PCR with primers designed to amplify four subclasses of IgG,we detected ? transcript variation in HeLaS3 after IL-2 stimulation at mRNA level.Results:The result of immunofluorescence-FACS showed that after IL-2 stimulation for 48 hours,membrane and cytoplasmic IgG expression elevated in HeLaS3.The result of Western blot showed after IL-2 stimulation for 48 hours,IgG expression elevated in HeLaS3.Meanwhile,the result of RT-PCR showed that ?1 transcript mildly elevated after IL-2 stimulation for 48 hours.Conclusion:IL-2 can promote IgG expression in cervical cancer cell line HeLaS3.
