Ecdysterone induces angiogenesis and protects neurologic function after focal cerebral ischemia in rats
- VernacularTitle:蜕皮甾酮对局灶性脑缺血大鼠血管生成的影响
- Author:
Chunxia LUO
;
Kangning CHEN
;
Hua FENG
;
Kaizhi LU
;
Lusi LI
- Publication Type:Journal Article
- Keywords:
ecdysterone;
focal cerebral ischemia;
vascular endothelial growth factor;
angiogenesis;
neurologic function
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of ecdysterone(EDS)on the level of VEGF protein in the brain,angiogenesis and neurologic function after focal cerebral ischemia in rats.Methods Rat with focal cerebral ischemia were established by occluding their middle cerebral artery.The established rats(n=36)were randomly and equally divided into EDS treatment group and ischemia group.EDS(20 mg?kg-1?d-1 for 7 d)was intraperitoneally injected into the rats of EDS treatment group 2 h after operation,and the animal of ischemia group received an intraperitoneal injection of the same solvent as in EDS group.Another 6 rats served as normal control.Rats were sacrificed in 7,14 and 21 d after operation,and the VEGF protein level and microvessel density(MVD)was detected with immunohistochemical methods and analyzed quantitatively with image system.Effect of EDS on neurologic recovery following brain ischemia were assessed using the neurologic severity scores(NSS).Results VEGF expression was not seen in normal control,and was higher in ischemia group than in the EDS treatment group at day 7 and 14,but the significant difference was only observed at day 7(P
