Reduced glutathione protects retina and optic nerve of rats after optic neuritis
- VernacularTitle:还原型谷胱甘肽对实验性视神经炎视神经和视网膜的影响
- Author:
Mengting LU
;
Pinghua LI
- Publication Type:Journal Article
- Keywords:
experimental autoimmune encephalomyelitis;
optic nerve;
retinal ganglion cells;
apoptosis;
malondialdehyde;
glutathione peroxidase;
nitric oxide;
glutathione
- From:Journal of Third Military Medical University
2003;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of reduced glutathione(rGSH)on the retina and optic nerve in rats with experimental optic neuritis,and the underlying mechanism.Methods Animal model of experimental allergic encephalomyelitis(EAE),which was as used for acute demyelinating optic neuritis were established in 48 female SD rats,and then the rats were randomly divided into EAE group(n=18)and GSH group(n=30).Another 6 female SD rats served as normal control.Rats of GSH group received an intraperitoneal injection of 100 mg?kg-1?d-1 GSH for 14 d from the beginning of immunization,while the rats of EAE group were given saline alternatively.To observe pathological changes in the optic nerve,retinal ganglion cells(RGCs)apoptosis,and measure malondialdehyde(MDA),glutathione peroxidase(GSH-PX),nitric oxide(NO)levels in the retina were observed and measured in the early period,peak period and recovery period(12 to 14 d,16 to 18 d,or 26 to 28 d after the immunization)of the neuritis.Results There were 18 rats(60%)from GSH group having the disease with a symptom score of 1 to 3.Compared with those of EAE group,their optic nerve fibers were in a comparatively good order,and more glial cells and not obvious demyelination were observed in morphology;Significant lesser apoptotic RGCs were observed in GSH group than in EAE group at the early and peak period(P
