The Detection of RhD? pseudogene and RhD-CE-D Hybridization Gene and Medicine Application in Rh-negative ones of Kunming Area
- VernacularTitle:昆明地区Rh阴性者RhD假基因(RhD ?)和杂交RhD-CE-D基因的检测和医学应用
- Author:
Zhaogang LIU
;
Shurong ZHONG
;
Liping HU
;
Bingying XU
;
Qiang JING
- Publication Type:Journal Article
- Keywords:
RhD(-)blood group;
D gene;
PCR-SSP;
RhD ?;
RhD-CE-D
- From:
Journal of Medical Research
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the D gene polymorphism of Rh-negative blood group in Kunming resident population with PCR technology for gene typing,and to establish and perfect PCR detecting method for RhD gene.Method 46 samples of Rh-negative blood samples were collected and PCR amplification with exon-SSP had been done firstly.The samples of D gene part existing were screened,then PCR amplifying by intron-SSP had been conducted.The PCR amplification production with intron-SSP was used to DNA sequence.Results Among 46 Rh-negative samples,the number of D gene lost intacfly was 29;the complete existing one with all exons was 12.The sequencing results were that 2 samples of only containing D10 were RhD-CE(3-9)-D and the sample with only D4 and D6 was the newly discovered variant allele RhD-CE(2-3,5,7-9)-D.RhD ? was not found in our study.Conclusion(1)There is high frequency of RhD-CE(3-9)-D in Kunming area.(2)A new variant allele of RhD-CE(2-3,5,7-9)-D is first found in Kunming area.
