Establishing model of polymyositis with damp-heat syndrome
- VernacularTitle:多发性肌炎湿热证模型的制作
- Author:
Minghui LAI
;
Youzhang LIU
- Publication Type:Journal Article
- Keywords:
Polymyositis;
Animal model;
Damp-heat syndrome;
Guinea pig;
Experimental study
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To research the method of Establishing model of polymyositis with damp-heat syndrome. Methods: 45 guinea pigs were randomly divided into three groups, group A (normal group), group B (experimental myositis group, control group), Group C (damp-heat group). In the control group, the rabbit skeletal muscle homogenate Garver,s complete adjuvant for subcutaneous immunization was used to injecting into the original guinea pigs. Besides the above-mentioned immune method, the heat-damp model method was used (feigan diet, hot and humid environment and pathogenic micro-organisms and other complex factors) to establish the polymyositis model with damp-heat syndrome. The general situations, such as the serum muscle enzymes, light microscopy, electron microscopy changes in each group were observed. Results: The results showed that the clinical performance, serum creatase, the pathological changes of skeletal muscle, lungs, liver of guinea pigs in damp-heat group were similar to the human polymyositis with damp-heat syndrome. Conclusion: The model can be used to study the human polymyositis with damp-heat syndrome, and provide the pathogenic and theoretical basis for treating polymyositis by TCM.
