Remodeling features of small intramyocardial arteries in rats with idiopathic hypertension
- VernacularTitle:自发性高血压大鼠心肌壁内小动脉的重构特征观察
- Author:
Huashan HONG
;
Jinshou WU
;
Qiong JIANG
- Publication Type:Journal Article
- Keywords:
rats,inbred SHR;
myocardium;
arterioles;
remodeling;
myocytes,smooth muscle;
cell proliferation;
apoptosis
- From:
Medical Journal of Chinese People's Liberation Army
1981;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the features of remodeling of intramyocardial small arteries(IMSAs) in rats with idiopathic hypertension(SHR).Methods12-week-old male SHR rats(experimental group,n=10) with the age and sex matched Wistar Kyoto rats(WKY,control group,n=10) were included in present study.Experiments were continued for 18 weeks.The blood pressure of all the animals was measured at the beginning and at the end of experiment,and then all the animals were sacrificed.The left ventricle tissue sections were stained with HE,Sirius-red,elasticity fibrin and collagen fibrin double staining(P/VB) for morphological evaluation of IMSAs.The wall area(WA),luminal area(LA) and percent wall area(%WA) of IMSAs were assessed with the compute-assisted image analysis system.The vascular smooth muscle cells(VSMCs) were recognized by ?-SMA immunohistochemical staining,the proliferation and apoptosis of IMSAs were demonstrated by proliferating cell nuclear antigen(PCNA) immunohistochemical staining and TdT-mediated dUTP nick-end labeling(TUNEL) technique,respectively.The apoptosis index(AI) and proliferation index(PI) were calculated respectively.Results Systolic blood pressure(SBP) in SHR group at week 12 and week 30 were significantly higher than those in WKY group(172.00?9.78mmHg vs 123.40?5.10mmHg,200.50?14.15mmHg vs 116.30?9.38mmHg,respectively,P
