Dysfunction of releasing adrenaline in primary cultured AMCC due to functional reduncdency primed by nerve growth factor.
- VernacularTitle:神经生长因子启动肾上腺髓质细胞功能冗余性致肾上腺素释放障碍的研究
- Author:
Jun WANG
;
Chengping HU
;
Juntao FENG
- Publication Type:Journal Article
- Keywords:
nerve growth factor;
adrenaline medullary chromaffin cells(AMCC);
reduncdency;
adrenaline
- From:
Chinese Journal of Practical Internal Medicine
2001;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the possible mechanism of the biologic ethology effects of NGF and SP(substance P)on primary cultured adrenaline medullary chromaffin cells(AMCC).Methods To establish primary cultured AMCC by means of enzyme digestion and purify the cells by means of isopycnic gradient centrifugation and differential plating.To observe the morphological and ultrastructural changes after the addition of NGF and SP,and detect the concentration of adrenaline and noradrenaline in serum by ELISA.Results Confervaceous processes could be observed after 2 d of addition of NGF to the culture and the processes strenched longer as days went by the observation of electron microscopy.there are some drumstick-like and villiform processes in the cell membrane and some vesiculation be observed near the cell membrane of the primary cultured AMCC cells after the addition of NGF.The bioblast was abundant but the structure was not clear in the intracytoplasm and the concentration of adrenaline were decreased(P
