Combination of erythropoietin with granulocyte-colony stimulating factor protects neonatal rat cardiomyocytes from hypoxia in vitro
- VernacularTitle:促红细胞生成素联合粒细胞集落刺激因子对乳鼠缺氧心肌细胞的保护作用
- Author:
Zhenhong FU
;
Wei DONG
;
Luyue GAI
- Publication Type:Journal Article
- Keywords:
hypoxia;
cardiomyocyte;
erythropoietin;
granulocyte-colony stimulating factor;
cell apoptosis
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the potential protective effects of erythropoietin (EPO) in combination with granulocyte-colony stimulating factor (G-CSF) on hypoxia cardiomyocytes. Methods After left ventricular cardiomyocytes were isolated from neonate rat, the cells were inoculated in a cultural atmosphere of 95%N2 and 5%CO2 for 24 h to establish hypoxia cardiomyocytes model. The protective effects of EPO and G-CSF at different concentrations were evaluated for the optimal concentration. Then the protective effects of EPO in combination with G-CSF were investigated under the optimized concentrations. The survival, apoptotic and necrotic rates of cardiomyocytes were assessed by flow cytometry. Results The mortality and ratio of apoptotic cells to total necrotic cell were higher in hypoxia cardiomyocytes than normal cells significantly (26.73% vs 5.63%,70.05% vs 37.83%, P0.05), and the combination group was much better than EPO and G-CSF alone groups (P
