Distribution of interstitial cells of Cajal in normal guinea pig upper urinary tract and its effect on smooth muscle contraction
- VernacularTitle:ICC样细胞在正常豚鼠上尿路的分布及对平滑肌收缩的影响
- Author:
Xiao ZHONG
;
Weibing LI
;
Qiang FANG
;
Bo SONG
- Publication Type:Journal Article
- Keywords:
interstitial cells of Cajal;
c-kit receptor;
upper urinary tract
- From:Journal of Third Military Medical University
1988;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution of interstitial cells of Cajal (ICCs) —the pacemaker cells of the gut in the different parts of guinea pig pyeloureteral tract and explore the effect on the contraction of smooth muscle. Methods The ureteropelvic junction, upper ureteral, middle ureteral, lower ureteral tissues harvested from 10 guinea pigs were performed KIT immunofluorescent staining in stretched preparations. Smooth muscle strips of 2 mm?6 mm were dissected from the sites for tone determination. The frequency and amplitude of the smooth muscle were compared among different sites of pyeloureteral tract. Results Cells with positive staining by KIT and with similar morphology to ICCs were found in the entire pyeloureteral tract of guinea pigs. The density of ICCs-like cells in the ureteropelvic junction, upper ureteral, middle ureteral, and lower ureteral tissues were (5.20?0.98), (3.90?0.98), (3.03?0.98) and (2.50?0.81) respectively. The contraction of strips in different sites varied according to the distribution of ICCs. The amplitude and frequency of strips from the ureteropelvic junction and upper ureteral were significantly higher than those from middle ureteral and lower ureteral (P
