Regulation of calcium current by emodin in guinea pig gallbladder smooth muscle
- VernacularTitle:大黄素对豚鼠胆囊平滑肌L型钙电流的调节
- Author:
Zhixuan WU
;
Baoping YU
;
Long XU
;
Hong XIA
;
Hesheng LUO
- Publication Type:Journal Article
- Keywords:
emodin;
gallbladder smooth muscle;
L-type calcium current;
patch clamp
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of emodin on the contraction of gallbladder smooth muscle(GBSM)and the L-type calcium current in GBSM cells.Methods Gallbladder muscle strips were obtained from adult guinea pigs and the resting tension was recorded.Gallbladder smooth muscle cells were isolated by enzymatic digestion,and calcium current was recorded by the whole-cell patch clamp method.Results Emodin-induced contraction of GBSM was significantly attenuated by pretreatment with nifedipine.Emodin increased the L-type calcium current in a dose-dependent manner.When 10 ?mol?L-1 emodin was applied to GBSM cells,the amplitude of L-type calcium current at +10 mV was enhanced by(45.2?2.26)%.In the presence of PKC inhibitor,staurosporine,emodin did not significantly affect the calcium current.Conclusion Emodin enhances L-type calcium current via PKC-dependent pathway and promotes gallbladder contraction.
