The regulation of Curcumin on the amyloidogentic pathaway of APP in Alzheimer's disease
- VernacularTitle:姜黄素对阿尔采末病中APP淀粉样酶切途径的调控作用
- Author:
Hongmei ZHANG
;
Xiong ZHANG
;
Yu LI
- Publication Type:Journal Article
- Keywords:
Alzheimer's disease;
curcumin;
APP;
BACE1;
A?
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Curcumin on the APP processing in the amyloidogenic pathway in vitro and explore its mechanisms on A? generation inhibition.Methods Plasmids APPswe and BACE1-mychis were transiently co-transfected in SH-SY5Y cell by LipofectaminTM2000.The cell line then treated with Curcumin at 0,1.25,5,20 ?mol?L-1 for 24 h,or with Curcumin at 5 ?mol?L-1 for 0,12,24 and 48 h for the time course assay.RT-PCR were performed to measure the endogenous levels of APP and BACE1 mRNA.Western blot were used to detect the protein expression of BACE1 and C99,the major ?-secretase cleavage product.The concentration of A?40/42 was detected by ?-amyloid 1~40 or 42 Colorimetric ELISA.Results RT-PCR results showed that the mRNA levels of APP and BACE1 were decreased obviously in a dose-and time-dependent manner after treated with Curcumin in SH-SY5Y(P
