The effect of Rehmannia glutinosa oligosaccharides on the damage induced by glutamate in hippocampal neurons
- VernacularTitle:地黄寡糖对谷氨酸诱导海马神经元损伤的影响
- Author:
Jialin SHI
;
Jing YANG
;
Xinli XU
;
Hongxin WANG
;
Ying JIN
;
Chunna LIU
- Publication Type:Journal Article
- Keywords:
ROS;
glutamate;
hippocampus;
neurons;
apoptosis
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of Rehmannia glutinosa oligosaccharides(ROS)on the damage induced by glutamate in hippocampal neurons.Methods The neurons isolated from hippocampus in new born SD rats were cultured for 7~9 days,which were specifically stained with NSE and then randomly divided into four groups:(Ⅰ)Normal cultures(control);(Ⅱ)ROS control cultures;(Ⅲ)Glutamate-exposed control cultures;(Ⅳ)Glutamate-exposed cultures pretreated with ROS.The neurons morphology was observed under inverted microscope;cell viability was assayed by MTT staining;LDH release was detected with chromatometry and flow cytometric analysis for identification and quantification of cell apoptosis.Results Compared with normal group,after exposure of glutamate for 24 h,the viability of neurons was decreased,LDH release and cell apoptosis were increased(P
