Ation mechanism of dihydroartemisinin combined with COX-2 inhibitor in S180 carcinoma
- VernacularTitle:二氢青蒿素联合环氧化酶2抑制剂抗S180肉瘤作用机制研究
- Author:
Jiali ZHANG
;
Zeng WANG
;
Beibei ZHUANG
;
Huijun ZHOU
- Publication Type:Journal Article
- Keywords:
dihydroartemisinin;
COX-2 inhibitor;
VEGF;
S180 sarcoma
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To observe the inhibitory effect of dihydroartemisinin combined with COX-2 inhibitor on S180 sarcoma and their possible antitumor mechanisms.Methods The ICR mice model of the subcutaneous transplanting tumor was established by S180 sarcoma to investigate the treatment effect of dihydroartemisinin and COX-2 inhibitor.Expressions of VEGF,COX-2 and CD31 in S180 sarcoma were detected and the amount of leukocyte was also observed.Results Dihydroartemisinin combined with COX-2 inhibitor could potently inhibit tumor growth and inflammation.The maximal antitumor rate reached 55.38%.The results of immunohistochemistry showed that COX-2 and VEGF protein expressions were weakened by dihydroartemisinin and COX-2 inhibitor,versus the control groups.Further,RT-PCR analysis showed that the expression of VEGF mRNA was also effectively decreased.Finally,a significant down-regulation effect of COX-2 and VEGF protein expression was observed.Conclusions Dihydroartemisinin combined with COX-2 inhibitor can significantly inhibit S180 tumor.The antitumor mechanism of these two drugs might be closely related to the effect of COX-2 and VEGF expression suppression.
