Correlation of enhancement radiosensitization of emodin isolated from Guangxi P. multiflorum Thunb on hypoxic nasopharyngeal cancer cells with expression of DNA repair genes
- VernacularTitle:大黄素对乏氧鼻咽癌细胞的放射增敏性与DNA修复基因的关系
- Author:
Ying LIU
;
Huaxin HOU
;
Danrong LI
;
Daohai CHENG
;
Yan LUO
- Publication Type:Journal Article
- Keywords:
emodin;
hypoxia;
repair gene;
real-time fluorescence quantitative RT-PCR;
nasopharyngeal carcinoma
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the effects of emodin isolated from Guangxi P.multiflorum Thunb on the expression of KU70/KU80 in hypoxic nasopharyngeal cancer CNE-1 cells and reveal the relationship between radiosensitization of emodin monomer and DNA repair genes.Methods The expression of hypoxia inducible factor-1?(HIF-1?)and DNA double-strand break repair genes(KU70/KU80)between the experimental groups and the control group under hypoxic condition was detected by the real-time fluorescence quantitative RT-PCR.Results Expression of HIF-1? was significantly increased under hypoxia condition.HIF-1? had no change after treatment with emodin alone.The expression level of KU70/KU80 was induced by radiation.Compared with radiation alone group,radiation combined hypoxia group obviously enhanced the expression of KU70/KU80.KU70/KU80 mRNA expression significantly reduced after radiation combined with emodin under hypoxic condition.Conclusion In the hypoxic environment,emodin combined with radiotherapy can effectively inhibit the expression of HIF-1 ? and DNA double-strand break repair genes(KU70/KU80),which may be its mechanism of radiosensitization.
