Establishment and application of screening method for DPPIV inhibitors in vitro
- VernacularTitle:二肽基肽酶Ⅳ抑制剂体外筛选模型的建立及应用
- Author:
Lei MIAO
;
Hongyu XU
;
Jianyong LEI
;
Jian JIN
;
Zhenghong XU
- Publication Type:Journal Article
- Keywords:
DPPIV inhibitor;
colorimetry
- From:
Chinese Pharmacological Bulletin
1987;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To develop a model for screening DPPIV inhibitor from Chinese Herbal medicine.Methods With Gly-Pro-PNA as a substrate,DPPIV activity was assayed by the chromogenic substrate.The concentration of DPPIV and Gly-Pro-PNA,temperature,pH and reaction time were optimized.Results The optimal enzymatic reaction system was as follows:DPPIV 0.5 U?L-1,Gly-Pro-PNA 0.262 ?mol?L-1,37℃,pH 8.2,reaction for 60 min.Further more,many kinds of herbal abstracts were screened and some had the DPPIV inhibitory function,such as Leech,Poria cum Radix Pini and Buddleja officinalis Maxim.Conclusion The model can be used to screen for DPPIV inhibitors in vitro.
