Expression of Macrophage Migration Inhibitory Factor in Lungs of Rats with Experimental Pulmonary Fibrosis.
- VernacularTitle:巨噬细胞移动抑制因子在肺纤维化大鼠肺组织中的表达
- Author:
Hua XIAO
;
Ping ZHANG
- Publication Type:Journal Article
- Keywords:
Pulmonaryfibrosis;
MIF;
Rat
- From:
Journal of Medical Research
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of macrophage migration inhibitory factor(MIF)in lung tissues of rats with experimental pulmonary fibrosis and to discuss its effects on pulmonary fibrosis.Methods36 male SD rats were randomly divided into two groups:the pulmonary fibrosis group and the control group.Rat pulmonary fibrosis was induced by an intratracheal injection of bleomycin.The following tests were undedaken: HE and Masson staining of lung section,immunohistochemical staining of protein of MIF in the lung,RT-PCR of MIFmRNA in the lung.ResultsCompared with the control group,the mRNA and protein expression of lung MIF on day 7 to 28 in pulmonary fibrosis increased and reached the peak on day 14 after an intratracheal injection of bleomycin(P
