Effect of sinomenine on expression of TLR2 and TLR4 of dendritic cells in rheumatoid arthritis
- VernacularTitle:青藤碱对类风湿关节炎患者外周血树突状细胞TLR2和TLR4表达的影响
- Author:
Keqiang YU
;
Ren LUO
- Publication Type:Journal Article
- Keywords:
Rheumatoid arthritis;
Sinomenine;
Dendritic cell;
Toll like receptor
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of sinomenine on expression of toll like receptor(TLR) 2 and TLR4 of dendritic cells in patients with rheumatoid arthritis in vitro.Methods: The peripheral blood mononuclear cells were isolated from 10 rheumatoid arthritis patients and differentiated into dendritic cells by stimulating with cytokines.Dendritic cells were exposed to high(5mM),middle(2mM),low(1mM) concentration sinomenine and control medium.TLR2 and TLR4 mRNA were detected by real time PCR.Western blot was used to measure the expression of TLR2 and TLR4.Results: In contrast to control,signifi cant lower expression of TLR2,TLR4 mRNA and protein were found in dendritic cells treated with sinomenine,especially in high and middle concentration.Conclusion: The therapeutical effect of sinomenine on rheumatoid arthritis is probably by inhibiting dendritic cells expressing TLR,which can transduce the in? ammatory signal.
