Identification of a Major Cytoplasmic Laminin Binding Protein as the Heat Shock Cognate Protein 70 in Highly Metastatic Human Colon Carcinoma Cells.
- Author:
Hee Jong WOO
- Publication Type:Original Article
- Keywords:
Hsc70;
Laminin binding protein;
Colon carcinoma
- MeSH:
Carrier Proteins*;
Cell Differentiation;
Chromatography, Affinity;
Colon*;
Cytoplasm*;
Gelatin;
Hot Temperature*;
Humans*;
Laminin*;
Neoplasm Metastasis;
Shock*
- From:Korean Journal of Immunology
1998;20(1):75-80
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
"It is not clear yet how the laminin is made in cytoplasm and is secreted by cells, though the significance of the interaction between laminin and cells during the development, cell differentiation, tumor growth, and metastasis is well known. Furthermore, it has been reported that aberrant expression. and secretion of laminin in malignant tumor cells, In this study I found a major cytoplasmic laminin binding protein (LBP), which was revealed as the heat shock cognate protein 70 (hsc70), in human colon carcinoma cells by cell surface labeling with '""I, laminin and gelatin affinity chromatography, peptide microsequencing, and immunoblot experiments. This suggests another function of hsc70 as the cytoplasmic LBP, which may play an important role in biosynthesis, assembly, and secretion of laminin in tumor cells."
