Effect of Radix Paeoniae Rubra on intimal proliferation and expression of MKP-1 mRNA after rabbit carotid artery injury
- VernacularTitle:中药赤芍对损伤介导的内膜增殖及MKP-1基因表达的影响
- Author:
Huimin ZHU
;
Lifen LIU
;
Zhe ZHENG
;
Jinxiu FAN
- Publication Type:Journal Article
- Keywords:
Radix Paeoniae Rubra;
Balloon injury;
Intimal proliferation;
MKP-1;
AgⅡ
- From:
China Journal of Traditional Chinese Medicine and Pharmacy
2005;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe the effect of Radix Paeoniae Rubra on intimal proliferation,activating of angiotensin Ⅱ(AgⅡ) and expression of Mitogen-activated protein kinase phosphatase-1(MKP-1) after carotid artery balloon injury in cholesterol-fed rabbits.Methods: Male rabbits were randomly divided into Radix Paeoniae Rubra(PPR) groups: high dose group(75、50、25g.kg-1.d-1;n=8),middle dose group(2g.kg-1.d-1),low dose group(1g.kg-1.d-1;n=8) and control group.Both groups received high fat forage(2% cholesterol + 5% lard).Balloon injury of carotid artery was performed.Carotid artery were harvested at the end of 10 weeks.Expression level of AgⅡwas measured by radioimmunoassay.MKP-1 expression was determined by RT-PCR.Immunohistochemical staining and morphological detection were adopted.Results: Compare with control group,expression of AgⅡ decreased obviously(P
