Expression and significance of cell apoptosis in the development of human embryonic spinal cord as detected by TUNEL staining
- VernacularTitle:原位缺口末端标记法检测人胚胎脊髓发育过程中细胞凋亡的表达及意义
- Author:
Xuehong LIU
;
Yong ZHANG
;
Jinping ZHANG
;
Aiyue YU
- Publication Type:Journal Article
- Keywords:
spinal cord;
apoptosis;
embryo;
humans;
in situ nick-end labeling
- From:
Medical Journal of Chinese People's Liberation Army
1982;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the regularity of cell apoptosis in the early development of human embryonic spinal cord tissue. Methods The apoptotic cells in the spinal cord tissue of human embryos were stained in the second, third and fourth month of gestation by terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) method, and the integral optical density (IOD) was analyzed with Nikon imaging system (NIS-DR). One-Way ANOVA was applied to compare these IODs. Results The positive expression of cells apoptosis was detected in the central canal, anterior horn and posterior horn of human embryonic spinal cord in the second, third and fourth month of gestation. The IOD values of apoptotic cells at the 3 time points were 134.9954?10.53257, 149.0331?5.66187 and 140.7892?7.65320, respectively. The IOD value of apoptotic cells of human embryonic spinal cord at the third month of fetal age was higher than that of the second and the fourth month of fetal age, and the IOD value at the fourth month of fetal age was higher than that of the second month of fetal age. With the advancement of fetal age, the expressions of IOD of apoptotic cells showed an up then down trend. One-Way ANOVA was employed to compare the IOD values of the apoptotic cells detected in the 3 fetal periods, and the results showed significant difference (P
