Inhibitory effect of circular dumbbell decoy ODNs targeting NF-?B on TNF-? expression in cortical neurons under oxygen glucose deprivation/reoxygenation
- VernacularTitle:NF-?B靶向性哑铃形“圈套”ODNs抑制大脑皮质神经元氧糖剥夺/复氧后TNF-?的表达
- Author:
Lanying HE
;
Yong LUO
- Publication Type:Journal Article
- Keywords:
NF-?B decoy ODNs;
cortical neurons;
oxygen glucose deprivation/reoxygenation;
TNF-?
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the inhibitory effects of circular dumbbell decoy oligodeoxynucleotides targeting NF-?B on TNF-? expression in cortical neurons under the oxygen glucose deprivation/reoxygenation(OGD/R).Methods The cultured neurons dissociated from the cortex of neonatal rats were observed on the 7th day after culture.To make the ischemia/reperfusion model in vitro,the neurons were exposed to OGD 4 h before cultured in normal culture media.The neurons were divided into 5 groups according to different processing methods 2 h before exposure to OGD,including normal group,OGD4 h/R6 h treated,NF-?B decoy ODNs treated,scrambled decoy ODNs treated and TransfastFastTM Transfection Reagent treated groups.The protein level of NF-?B P65 in primary neurons was detected by Western blotting.The protein and mRNA levels of TNF-? were detected by immunocytochemical method and RT-PCR,respectively.Results NF-?B decoy ODNs could effectively inhibit the expression levels of NF-?B P65 protein,TNF-? mRNA and protein in neurons(P
