Mechanism of protein kinase C? mediated multidrug resistance in human glioma cell line SHG-44
- VernacularTitle:蛋白激酶C?介导人神经胶质瘤细胞株多药耐药机制的研究
- Author:
Jin CHEN
;
Yuan CHENG
;
Jingyuan WAN
- Publication Type:Journal Article
- Keywords:
glioma;
protein kinase C?;
multidrug resistance
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To understand the molecular mechanism by which protein kinase C? mediates multidrug resistance of human glioma cell line SHG-44.Methods SHG-44/ADM was constructed by stepwise concentration increasing method and intermittent administration method.SHG-44/WT and SHG-44/ADM were treated by PKC? reactivator PMA and PKC? inhibitor staurosporine,then the expressions of PKC? and MDR-1 were detected by Western blotting,the PKC? activity was assayed by kinase,and ADM accumulation was determined by fluorescence spectrometry.Results PMA increased PKC? activity and MDR-1 protein expression and activity.Staurosporine was able to block PKC? activity and decrease MDR-1 expression and activity.Conclusion Multidrug resistance in human glioma cells is mediated by PKC? via MDR-1 pathway.
