Histomorphology,cellular biocompatibility and tensile force resistance property of fibrin-binding amniotic membrane in vitro
- VernacularTitle:胶联羊膜的组织形态、细胞相容性及抗拉力特性研究
- Author:
Ning KE
;
Min ZHAO
;
Zhen LI
- Publication Type:Journal Article
- Keywords:
fibrin-binding amniotic membrane;
histomorphology;
carrier;
elastic modulus
- From:Journal of Third Military Medical University
1983;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the histomorphology,cellular biocompatibility in vitro and tensile force resistance property of fibrin-binding amniotic membrane(FBAM)and to explore its feasibility and superiority using as ocular surface graft.Methods After observing the morphology of FBAM under light microscope and scanning electron microscope,we did primary culture of corneal epithelial cells,taking monolayer fibrin-binding amniotic membrane as carrier,then observed cell growth overlay area.The elongation nature of FBAM was tested on electronic universal testing machine by calculating elastic modulus,using bilayer amniotic membrane(BAM)and monolayer amniotic membrane(MAM)as controls.Results The fresh FBAM was integrated as a whole,with structurally complete fibrin and amniotic membrane as well as tight binding of fibrin to amniotic membrane.The reserved FBAM was structurally complete,brittler than the fresh FBAM,while amniotic membrane still bound tightly to the fibrin,but under microscope the fiber reticular formation was fairly fuzzy.Single layer of epithelial cells was formed on FBAM after about seven-day culture in vitro.Disparity of cell growth overlay area was significant(P
