DETERMINATION OF POLYUNSATURATED FATTY ACID IN BREAST MILK BY HPLC
- VernacularTitle:高效液相色谱法测定母乳多不饱和脂肪酸
- Author:
Yujuan HUANG
;
Haiqi LI
- Publication Type:Journal Article
- Keywords:
PUFA;
HPLC;
breast milk
- From:
Acta Nutrimenta Sinica
1956;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop HPLC method for determination of polyunsaturated fatty acid(PUFA) in breast milk.Method PUFA were extracted from breast milk with mixture of methanol: N-hexane:phosphoric acid(15:25:3).PUFA were derived by phenacyl bromide.The mobile phase was acetonitrile:water(81:19,v/v) at 1.0 ml/min with UV detection at 252 nm and column temperature was 35℃.The analytic column was Hypersil BDS C18(4.6mm?150mm,5 ?m).Results The average recovery was(98?5)% and the relative standard deviation of intra-day and inter-day were limited within 5.9%.Conclusion The method was stable,sensitive and accurate,and suitable for determination of PUFA in breast milk.
