Protective effect of extract of astragalus against injury induced by hypoxia/reoxygenation in hippocampus neuron
- VernacularTitle:黄芪提取物对缺氧/复氧诱导神经元损伤的保护作用
- Author:
Fenfang ZHU
;
Yanyan YIN
;
Weiping LI
;
Weizu LI
;
Guocui WU
;
Huilin GONG
;
Wen ZHANG
- Publication Type:Journal Article
- Keywords:
EA;
cell culture;
hypoxia/reoxygenation;
signal transduction
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To study the effect of EA on the injury induced by hypoxia/reoxygenation in primary cultures of rat hippocampal neurons.Methods Rat hippocampal neurons in primary culture were used,and a apoptosis model was induced by hypoxia/reoxygenation.MTT assay and LDH releasing rate were used to detect the cell viability.The apoptosis rate of hippocampal neurons was analyzed by Hoechst 33258 staining,flow cytometry with AnnexinV-FITC and PI staining.Western blot was used to detect the protein expression of AKT and p-AKT.Results Compared to control group,three hours of hypoxia followed by sixteen hours of reoxygenation induced hippocampal neuronal apoptosis.EA could raise the neuronal viability and reduce apoptosis rate and the damage degree of rat hippocampal neurons.EA could increase the expressing of p-AKT.Conclusions EA has protective effects on damaged neurons,and the mechanism may be related to activating the PI3K-AKT signal transduction pathway.
