Effects of different conserve time on physiological characteristics of allograft nerve transplantation
10.3969/j.issn.1671-8348.2013.29.022
- VernacularTitle:不同冷冻时间对异体神经移植后生理特性的影响
- Author:
Yong ZHANG
;
Delong CHEN
;
Xiaolong CHEN
;
Xiyin ZENG
;
Chunfu NIE
- Publication Type:Journal Article
- Keywords:
cryopreservation;
allograft nerve;
freezing;
transplantation;
physiological characteristics;
rate,Wistar
- From:
Chongqing Medicine
2013;(29):3521-3523,3526
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of different conserve time in ultra deep cryopreservation (-196 ℃)on physiological characteristics of allograft nerve transplantation .Methods A total of 80 female Wistar rats were divide into three groups by random principle:derived group which 20 rats were sacrificed to get both sides of femoral nerves ;control groups which each group had 10 rats include group A for fresh autologous nerve transplantation ,group B for fresh allograft nerve transplantation ;experiment groups which each group had 10 rats including groups C ,D ,E ,F that transplanted after femoral nerves conserve in the -196 ℃ for 3 ,6 ,9 , 12 weeks respectively ,and the results of exterior appearance ,light microscope ,electron microscope were observed and electrophysi-ological test was conducted after transplantation .Results After 9 weeks transplantation :the physiological characteristics of group B was most affected ,followed by group C ,D ,E ,F ,group A was with minimal impact ;the result of electrophysiological test showed that groups A&B ,A&E ,A&F had significant difference(P<0 .05) .Conclusion The physiological characteristics of allograft nerve transplantation relate to the freezing time .
