Effects of cerebrolysin on intracellular calcium in primary cultured neurons after fluid percussion injury
- VernacularTitle:脑活素对液压冲击伤后原代神经细胞内游离钙的影响
- Author:
Huiling HUANG
;
Qiaoli WU
;
Chen WANG
;
Qiong WANG
;
Wenzhi ZHANG
;
Xin SU
- Publication Type:Journal Article
- Keywords:
cerebrolysin;
intracellular calcium;
neurons;
laser scanning confocal microscope
- From:
Chinese Pharmacological Bulletin
1987;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Aim To investigate the changes of intracellular calcium in primary cultured neurons after fluid percussion injury under the effects of cerebrolysin.Methods The neurons of rats were divided into: normal group,FPI group and cerebrolysin group(0 h and 1 h treatment after fluid percussion injury(FPI)).The intracellular calcium([Ca2+]i) at rats neurons in 24 h and 48 h postinjury were measured by using the laser scanning confocal microscope under calcium fluorescent indicator Fluo-3/AM.Results The [Ca2+]i at rats neurons were markedly increased after 24 h postinjury compared with normal neurons and maintained the higher level after 48 h.Cerebrolysin,whenever added at 0h or 1h after FBI,could significantly decrease the rise of [Ca2+]i on 24 h postinjury,which only happened in 48 h postinjury by 1 h treatment after FPI.Conclusion Cerebrolysin has the protective effects on primary cultured rat cortical neurons of rats and has the time-window treatment.
