Changes of PTEN gene expression in radiation-induced bystander cells
- VernacularTitle:PTEN在电离辐射诱导旁效应细胞中的表达变化
- Author:
Jian YANG
;
Ling HAN
;
Xian GAO
- Publication Type:Journal Article
- Keywords:
PTEN;
ionizing radiation;
bystander effect;
cell proliferation;
cell cycle
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study the changes of PTEN gene expression in radiation-induced bystander cells.Methods:The irradiated cells and non-irradiated cells were co-cultured in a ratio of 11.The culture medium of irradiated cells was used to culture non-irradiated cells to create two bystander cell models. The expression of PTEN mRNA and protein in the bystander cells,irradiated cells,non-irradiated cells was compared.Results:The protein expression of PTEN in the irradiated cells was negatively associated with the irradiation dose.The down-regulation degree of PTEN protein was related to the different treatments of cells.The most prominent down-regulation was found in the bystander cells cultured with medium of irradiated cells.The down-regulation of PTEN protein in the two kinds of bystander cells was more severe than that in the irradiated cells(P
