Regulatory mechanism of transforming growth factor-beta 1 on mechanical properties of tissue engineered heart valve
- VernacularTitle:TGF-?_1调控组织工程瓣膜力学性能机制研究
- Author:
Hao HONG
;
Nianguo DONG
;
Jiawei SHI
- Publication Type:Journal Article
- Keywords:
transforming growth factor-beta 1;
tissue engineering;
heart valve;
lysyl oxidase;
collagen;
mechanical properties
- From:
Academic Journal of Second Military Medical University
1982;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To observe whether transforming growth factor-beta 1 (TGF-?1) can improve the mechanical properties of tissue engineered heart valve (TEHV) prepared by TGF-?1 and myofibroblasts.Methods:Myofibroblasts were isolated,cultured,identified,and seeded onto the decellularized aortic valve leaflet.In the experimental group,TEHV were cultured with DMEM containing 10 ng/ml TGF-?1 for 14 days.In the control group,TEHV were cultured with DMEM only for 14 days.The contents of lysyl oxidase (LOX) and hydroxyproline,the expression of LOX and COLL-1 mRNA,and the mechanical properties of the TEHVs were analyzed in each group.Results:The contents of LOX and hydroxyproline,expression of LOX and COLL-1 mRNA,Max-load,and Max-stress were significantly higher in the experimental group than those in the control group (all P
