Effects of radiation on growth and CCN1 expression of mice fibroblast cell line L929
- VernacularTitle:辐射对L929细胞生长及CCN1基因表达的影响
- Author:
Yinghua WAN
;
Weike SI
;
Yejun DU
;
Zhaoquan LI
;
Jing PAN
;
Chen ZHAO
;
Jun LI
;
Yongping SU
- Publication Type:Journal Article
- Keywords:
radiation,ionizing;
fibroblasts,L929;
cysteine-rich 61
- From:Journal of Third Military Medical University
1983;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of radiation on the growth and expression of cysteine-rich 61(Cyr61/CCN1) of L929 cells and investigate the relationship between CCN1 expression and radiation injury.Methods L929 cells were cultured and divided into 2 groups,cells irradiated with 4 Gy ?-irradiation as radio-group and untreated cells as control group.The cell proliferation was measured by MTT assay and plate colony formation testing.Flow cytometry was utilized to quantify the cell cycle distribution.CCN1 expression at protein and mRNA levels were determined by immunocytochemistry(ICC) and RT-PCR respectively.Results Significant inhibition of proliferation(P
